Composite
tetR oprF*

Part:BBa_K1648049:Design

Designed by: Leung King Pong   Group: iGEM15_Hong_Kong-CUHK   (2015-05-31)


Mutated Av oprF induced by J13002


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 555


Design Notes

Insert mutated oprF into R0040 by standard restriction sites.

Source

R0040 is provided from iGEM distribution kits. Mutated oprF is synthesized by IDT.

References